chrebp antibody Search Results


chrebp  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology chrebp
Chrebp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chrebp antibody  (Novus Biologicals)
94
Novus Biologicals chrebp antibody
Chrebp Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chrebp  (Novus Biologicals)
95
Novus Biologicals chrebp
Chrebp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal antibody  (Novus Biologicals)
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Novus Biologicals rabbit polyclonal antibody
Rabbit Polyclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chrebp  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc chrebp
Chrebp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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anti chrebp antibody  (Novus Biologicals)
90
Novus Biologicals anti chrebp antibody
Figure 5. Glucose sensor <t>ChREBP</t> contributed to the metformin-mediated suppressing of the transcriptional expression <t>of</t> <t>PCSK9.</t> HepG2 cells were cultured in high glucose (25 mmol/L) or low glucose (5 mmol/L) medium for 6 h. A, Quantification and screening of the expression of glucose sensor-related transcription factors by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. B and C, HepG2 cells were cultured in high glucose (25 mmol/L) in the presence or absence of metformin (2 mmol/L) for 24 h. ChREBP (carbohydrate-responsive element-binding protein) expression was measured by qRT-PCR (B) and Western blotting (C). D, Immunoblot showing ChREBP protein expression in the cytosol and nuclear extracts isolated from the livers of PBS or metformin-treated C57BL/6J mice. Lamin A/C is nuclear loading control. E–G, HepG2 cells were pretreated with siControl or siChREBP for 24 h and incubated with a medium with or without 2 mmol/L metformin for another 24 h. Quantification of ChREBP (E) and PCSK9 (F) expression by qRT-PCR. Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Immunoblot showing ChREBP and PCSK9 protein expression (G). H, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h and then incubated in a medium containing 2 mmol/L metformin or PBS for another 20 h. Immunoblot showing ChREBP and PCSK9 protein expression. Values are mean±SEM. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. *P<0.05; **P<0.01, compared with PBS. Unpaired Student t test for A–C. Two-way ANOVA and Sidak multiple comparisons test for D–G.
Anti Chrebp Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti chrebp antibody/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
anti chrebp antibody - by Bioz Stars, 2026-04
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antichrebp  (Novus Biologicals)
92
Novus Biologicals antichrebp
Figure 5. Glucose sensor <t>ChREBP</t> contributed to the metformin-mediated suppressing of the transcriptional expression <t>of</t> <t>PCSK9.</t> HepG2 cells were cultured in high glucose (25 mmol/L) or low glucose (5 mmol/L) medium for 6 h. A, Quantification and screening of the expression of glucose sensor-related transcription factors by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. B and C, HepG2 cells were cultured in high glucose (25 mmol/L) in the presence or absence of metformin (2 mmol/L) for 24 h. ChREBP (carbohydrate-responsive element-binding protein) expression was measured by qRT-PCR (B) and Western blotting (C). D, Immunoblot showing ChREBP protein expression in the cytosol and nuclear extracts isolated from the livers of PBS or metformin-treated C57BL/6J mice. Lamin A/C is nuclear loading control. E–G, HepG2 cells were pretreated with siControl or siChREBP for 24 h and incubated with a medium with or without 2 mmol/L metformin for another 24 h. Quantification of ChREBP (E) and PCSK9 (F) expression by qRT-PCR. Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Immunoblot showing ChREBP and PCSK9 protein expression (G). H, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h and then incubated in a medium containing 2 mmol/L metformin or PBS for another 20 h. Immunoblot showing ChREBP and PCSK9 protein expression. Values are mean±SEM. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. *P<0.05; **P<0.01, compared with PBS. Unpaired Student t test for A–C. Two-way ANOVA and Sidak multiple comparisons test for D–G.
Antichrebp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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novus bio  (Novus Biologicals)
92
Novus Biologicals novus bio
Figure 5. Glucose sensor <t>ChREBP</t> contributed to the metformin-mediated suppressing of the transcriptional expression <t>of</t> <t>PCSK9.</t> HepG2 cells were cultured in high glucose (25 mmol/L) or low glucose (5 mmol/L) medium for 6 h. A, Quantification and screening of the expression of glucose sensor-related transcription factors by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. B and C, HepG2 cells were cultured in high glucose (25 mmol/L) in the presence or absence of metformin (2 mmol/L) for 24 h. ChREBP (carbohydrate-responsive element-binding protein) expression was measured by qRT-PCR (B) and Western blotting (C). D, Immunoblot showing ChREBP protein expression in the cytosol and nuclear extracts isolated from the livers of PBS or metformin-treated C57BL/6J mice. Lamin A/C is nuclear loading control. E–G, HepG2 cells were pretreated with siControl or siChREBP for 24 h and incubated with a medium with or without 2 mmol/L metformin for another 24 h. Quantification of ChREBP (E) and PCSK9 (F) expression by qRT-PCR. Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Immunoblot showing ChREBP and PCSK9 protein expression (G). H, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h and then incubated in a medium containing 2 mmol/L metformin or PBS for another 20 h. Immunoblot showing ChREBP and PCSK9 protein expression. Values are mean±SEM. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. *P<0.05; **P<0.01, compared with PBS. Unpaired Student t test for A–C. Two-way ANOVA and Sidak multiple comparisons test for D–G.
Novus Bio, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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horseradish peroxidase conjugated rabbit anti chrebp  (Novus Biologicals)
90
Novus Biologicals horseradish peroxidase conjugated rabbit anti chrebp
Figure 5. Glucose sensor <t>ChREBP</t> contributed to the metformin-mediated suppressing of the transcriptional expression <t>of</t> <t>PCSK9.</t> HepG2 cells were cultured in high glucose (25 mmol/L) or low glucose (5 mmol/L) medium for 6 h. A, Quantification and screening of the expression of glucose sensor-related transcription factors by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. B and C, HepG2 cells were cultured in high glucose (25 mmol/L) in the presence or absence of metformin (2 mmol/L) for 24 h. ChREBP (carbohydrate-responsive element-binding protein) expression was measured by qRT-PCR (B) and Western blotting (C). D, Immunoblot showing ChREBP protein expression in the cytosol and nuclear extracts isolated from the livers of PBS or metformin-treated C57BL/6J mice. Lamin A/C is nuclear loading control. E–G, HepG2 cells were pretreated with siControl or siChREBP for 24 h and incubated with a medium with or without 2 mmol/L metformin for another 24 h. Quantification of ChREBP (E) and PCSK9 (F) expression by qRT-PCR. Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Immunoblot showing ChREBP and PCSK9 protein expression (G). H, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h and then incubated in a medium containing 2 mmol/L metformin or PBS for another 20 h. Immunoblot showing ChREBP and PCSK9 protein expression. Values are mean±SEM. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. *P<0.05; **P<0.01, compared with PBS. Unpaired Student t test for A–C. Two-way ANOVA and Sidak multiple comparisons test for D–G.
Horseradish Peroxidase Conjugated Rabbit Anti Chrebp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti chrebp  (Novus Biologicals)
90
Novus Biologicals anti chrebp
Figure 5. Glucose sensor <t>ChREBP</t> contributed to the metformin-mediated suppressing of the transcriptional expression <t>of</t> <t>PCSK9.</t> HepG2 cells were cultured in high glucose (25 mmol/L) or low glucose (5 mmol/L) medium for 6 h. A, Quantification and screening of the expression of glucose sensor-related transcription factors by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. B and C, HepG2 cells were cultured in high glucose (25 mmol/L) in the presence or absence of metformin (2 mmol/L) for 24 h. ChREBP (carbohydrate-responsive element-binding protein) expression was measured by qRT-PCR (B) and Western blotting (C). D, Immunoblot showing ChREBP protein expression in the cytosol and nuclear extracts isolated from the livers of PBS or metformin-treated C57BL/6J mice. Lamin A/C is nuclear loading control. E–G, HepG2 cells were pretreated with siControl or siChREBP for 24 h and incubated with a medium with or without 2 mmol/L metformin for another 24 h. Quantification of ChREBP (E) and PCSK9 (F) expression by qRT-PCR. Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Immunoblot showing ChREBP and PCSK9 protein expression (G). H, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h and then incubated in a medium containing 2 mmol/L metformin or PBS for another 20 h. Immunoblot showing ChREBP and PCSK9 protein expression. Values are mean±SEM. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. *P<0.05; **P<0.01, compared with PBS. Unpaired Student t test for A–C. Two-way ANOVA and Sidak multiple comparisons test for D–G.
Anti Chrebp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti chrebp/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
anti chrebp - by Bioz Stars, 2026-04
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chrebpb  (Novus Biologicals)
92
Novus Biologicals chrebpb
Figure 5. Glucose sensor <t>ChREBP</t> contributed to the metformin-mediated suppressing of the transcriptional expression <t>of</t> <t>PCSK9.</t> HepG2 cells were cultured in high glucose (25 mmol/L) or low glucose (5 mmol/L) medium for 6 h. A, Quantification and screening of the expression of glucose sensor-related transcription factors by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. B and C, HepG2 cells were cultured in high glucose (25 mmol/L) in the presence or absence of metformin (2 mmol/L) for 24 h. ChREBP (carbohydrate-responsive element-binding protein) expression was measured by qRT-PCR (B) and Western blotting (C). D, Immunoblot showing ChREBP protein expression in the cytosol and nuclear extracts isolated from the livers of PBS or metformin-treated C57BL/6J mice. Lamin A/C is nuclear loading control. E–G, HepG2 cells were pretreated with siControl or siChREBP for 24 h and incubated with a medium with or without 2 mmol/L metformin for another 24 h. Quantification of ChREBP (E) and PCSK9 (F) expression by qRT-PCR. Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Immunoblot showing ChREBP and PCSK9 protein expression (G). H, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h and then incubated in a medium containing 2 mmol/L metformin or PBS for another 20 h. Immunoblot showing ChREBP and PCSK9 protein expression. Values are mean±SEM. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. *P<0.05; **P<0.01, compared with PBS. Unpaired Student t test for A–C. Two-way ANOVA and Sidak multiple comparisons test for D–G.
Chrebpb, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chrebpb/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
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Image Search Results


Figure 5. Glucose sensor ChREBP contributed to the metformin-mediated suppressing of the transcriptional expression of PCSK9. HepG2 cells were cultured in high glucose (25 mmol/L) or low glucose (5 mmol/L) medium for 6 h. A, Quantification and screening of the expression of glucose sensor-related transcription factors by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. B and C, HepG2 cells were cultured in high glucose (25 mmol/L) in the presence or absence of metformin (2 mmol/L) for 24 h. ChREBP (carbohydrate-responsive element-binding protein) expression was measured by qRT-PCR (B) and Western blotting (C). D, Immunoblot showing ChREBP protein expression in the cytosol and nuclear extracts isolated from the livers of PBS or metformin-treated C57BL/6J mice. Lamin A/C is nuclear loading control. E–G, HepG2 cells were pretreated with siControl or siChREBP for 24 h and incubated with a medium with or without 2 mmol/L metformin for another 24 h. Quantification of ChREBP (E) and PCSK9 (F) expression by qRT-PCR. Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Immunoblot showing ChREBP and PCSK9 protein expression (G). H, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h and then incubated in a medium containing 2 mmol/L metformin or PBS for another 20 h. Immunoblot showing ChREBP and PCSK9 protein expression. Values are mean±SEM. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. *P<0.05; **P<0.01, compared with PBS. Unpaired Student t test for A–C. Two-way ANOVA and Sidak multiple comparisons test for D–G.

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: New Insight Into Metformin-Induced Cholesterol-Lowering Effect Crosstalk Between Glucose and Cholesterol Homeostasis via ChREBP (Carbohydrate-Responsive Element-Binding Protein)-Mediated PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) Regulation

doi: 10.1161/atvbaha.120.315708

Figure Lengend Snippet: Figure 5. Glucose sensor ChREBP contributed to the metformin-mediated suppressing of the transcriptional expression of PCSK9. HepG2 cells were cultured in high glucose (25 mmol/L) or low glucose (5 mmol/L) medium for 6 h. A, Quantification and screening of the expression of glucose sensor-related transcription factors by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. B and C, HepG2 cells were cultured in high glucose (25 mmol/L) in the presence or absence of metformin (2 mmol/L) for 24 h. ChREBP (carbohydrate-responsive element-binding protein) expression was measured by qRT-PCR (B) and Western blotting (C). D, Immunoblot showing ChREBP protein expression in the cytosol and nuclear extracts isolated from the livers of PBS or metformin-treated C57BL/6J mice. Lamin A/C is nuclear loading control. E–G, HepG2 cells were pretreated with siControl or siChREBP for 24 h and incubated with a medium with or without 2 mmol/L metformin for another 24 h. Quantification of ChREBP (E) and PCSK9 (F) expression by qRT-PCR. Results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Immunoblot showing ChREBP and PCSK9 protein expression (G). H, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h and then incubated in a medium containing 2 mmol/L metformin or PBS for another 20 h. Immunoblot showing ChREBP and PCSK9 protein expression. Values are mean±SEM. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. *P<0.05; **P<0.01, compared with PBS. Unpaired Student t test for A–C. Two-way ANOVA and Sidak multiple comparisons test for D–G.

Article Snippet: Primary antibodies used in this study were obtained from the following sources: anti-PCSK9 (Abcam, 1:2000 working dilution), anti-ChREBP antibody (Novus, 1:1000 working dilution), anti-SREBP2 (sterol regulatory element-binding protein 2), antibody (Abcam 1:1000 working dilution), anti-β-ACTIN (Cell Signaling, 1:5000), and anti-GAPDH (Santa Cruz Biotechnology, Inc, 1:1000 working dilution).

Techniques: Expressing, Cell Culture, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Binding Assay, Western Blot, Isolation, Control, Incubation, Infection

Figure 6. Glucose sensor ChREBP (carbohydrate-responsive element-binding protein) is a novel regulator of PCSK9 (proprotein convertase subtilisin/kexin type 9) transcription expression in hepatocytes. A, Diagram and nucleotide sequences of the putative E-box ChREBP-binding site (ChoRE-like sequence) and of the mutation of the promoter region of human PCSK9 gene used for luciferase reporter constructs are shown. B, Huh 7 cells were infected with AdLacZ and AdChREBP for 16 h and then co-transfected with wild type or mutant PCSK9 promoter driving luciferase reporter plasmid and pRL-TK (thymidine kinase promoter-Renilla luciferase reporter) for 24 h. Dual luciferase report assays were used and pRL-TK was used as internal control. C, Quantitative ChIP assay was performed using HepG2 cells cultured in no glucose or high glucose (25 mmol/L) medium using IgG and ChREBP antibodies followed by amplification of PCSK9 promoter. Data were normalized to input fraction (n=3). D and E, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h to analyze the PCSK9 mRNA levels (D) and protein levels (E). P indicates premature PCSK9. M indicates mature PCSK9. F–H, AdLacZ or AdChREBP (5×108 pfu per mouse) were administered via tail vein injection to male C57BL/6 mice (n=9 per group). After 1 wk, mice were fasted overnight and then refed 6 h. F, Quantification of Pcsk9 by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) in the liver samples from C57BL/6J mice treated with AdLacZ or AdChREBP. (G) Representative immunoblots show PCSK9 and ChREBP protein expression in the livers from C57BL/6J mice treated with AdLacZ or AdChREBP. (H) Quantifications of serum PCSK9 levels from AdLacZ and AdChREBP-treated mice by ELISA kit. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. F–H, Data for biological duplicates±SEM. *P<0.05, **P<0.01, compared with control. Unpaired Student t test for D, F–H. Two-way ANOVA and Sidak multiple comparisons test for B, C, and E.

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: New Insight Into Metformin-Induced Cholesterol-Lowering Effect Crosstalk Between Glucose and Cholesterol Homeostasis via ChREBP (Carbohydrate-Responsive Element-Binding Protein)-Mediated PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) Regulation

doi: 10.1161/atvbaha.120.315708

Figure Lengend Snippet: Figure 6. Glucose sensor ChREBP (carbohydrate-responsive element-binding protein) is a novel regulator of PCSK9 (proprotein convertase subtilisin/kexin type 9) transcription expression in hepatocytes. A, Diagram and nucleotide sequences of the putative E-box ChREBP-binding site (ChoRE-like sequence) and of the mutation of the promoter region of human PCSK9 gene used for luciferase reporter constructs are shown. B, Huh 7 cells were infected with AdLacZ and AdChREBP for 16 h and then co-transfected with wild type or mutant PCSK9 promoter driving luciferase reporter plasmid and pRL-TK (thymidine kinase promoter-Renilla luciferase reporter) for 24 h. Dual luciferase report assays were used and pRL-TK was used as internal control. C, Quantitative ChIP assay was performed using HepG2 cells cultured in no glucose or high glucose (25 mmol/L) medium using IgG and ChREBP antibodies followed by amplification of PCSK9 promoter. Data were normalized to input fraction (n=3). D and E, HepG2 cells were infected with AdLacZ or AdChREBP for 24 h to analyze the PCSK9 mRNA levels (D) and protein levels (E). P indicates premature PCSK9. M indicates mature PCSK9. F–H, AdLacZ or AdChREBP (5×108 pfu per mouse) were administered via tail vein injection to male C57BL/6 mice (n=9 per group). After 1 wk, mice were fasted overnight and then refed 6 h. F, Quantification of Pcsk9 by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) in the liver samples from C57BL/6J mice treated with AdLacZ or AdChREBP. (G) Representative immunoblots show PCSK9 and ChREBP protein expression in the livers from C57BL/6J mice treated with AdLacZ or AdChREBP. (H) Quantifications of serum PCSK9 levels from AdLacZ and AdChREBP-treated mice by ELISA kit. qRT-PCR results are expressed as relative intensity normalized to the amount of housekeeping gene transcript and of controls. Western blot results are normalized by β-ACTIN. Three independent experiments with triplicates per experiment. F–H, Data for biological duplicates±SEM. *P<0.05, **P<0.01, compared with control. Unpaired Student t test for D, F–H. Two-way ANOVA and Sidak multiple comparisons test for B, C, and E.

Article Snippet: Primary antibodies used in this study were obtained from the following sources: anti-PCSK9 (Abcam, 1:2000 working dilution), anti-ChREBP antibody (Novus, 1:1000 working dilution), anti-SREBP2 (sterol regulatory element-binding protein 2), antibody (Abcam 1:1000 working dilution), anti-β-ACTIN (Cell Signaling, 1:5000), and anti-GAPDH (Santa Cruz Biotechnology, Inc, 1:1000 working dilution).

Techniques: Binding Assay, Expressing, Sequencing, Mutagenesis, Luciferase, Construct, Infection, Transfection, Plasmid Preparation, Control, Cell Culture, Amplification, Injection, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Figure 8. Proposed model of cross-talk between glucose and cholesterol homeostasis via ChREBP (carbohydrate-responsive element-binding protein)-mediated PCSK9 (proprotein convertase subtilisin/kexin type 9) expression. LDL-Cholesterol indicates low-density lipoprotein cholesterol.

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: New Insight Into Metformin-Induced Cholesterol-Lowering Effect Crosstalk Between Glucose and Cholesterol Homeostasis via ChREBP (Carbohydrate-Responsive Element-Binding Protein)-Mediated PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) Regulation

doi: 10.1161/atvbaha.120.315708

Figure Lengend Snippet: Figure 8. Proposed model of cross-talk between glucose and cholesterol homeostasis via ChREBP (carbohydrate-responsive element-binding protein)-mediated PCSK9 (proprotein convertase subtilisin/kexin type 9) expression. LDL-Cholesterol indicates low-density lipoprotein cholesterol.

Article Snippet: Primary antibodies used in this study were obtained from the following sources: anti-PCSK9 (Abcam, 1:2000 working dilution), anti-ChREBP antibody (Novus, 1:1000 working dilution), anti-SREBP2 (sterol regulatory element-binding protein 2), antibody (Abcam 1:1000 working dilution), anti-β-ACTIN (Cell Signaling, 1:5000), and anti-GAPDH (Santa Cruz Biotechnology, Inc, 1:1000 working dilution).

Techniques: Binding Assay, Expressing